Loud boos could be heard during a brief break in play to allow players observing Ramadan to break their fast during Saturday's Premier League game between Leeds United and Manchester City.
The 17:30 GMT kick-off at Elland Road was paused in the 13th minute as both sets of players came to the side of the pitch to take on fluids.
A message was displayed on the big screen inside the stadium stating: "As tonight's match takes place inside the holy month of Ramadan, play has been paused briefly to allow players to break their fast."
City had three Muslim players - Rayan Cherki, Rayan Ait-Nouri and Omar Marmoush - in their starting line-up, with Abdukodir Khusanov on the bench.
BBC Sport has contacted Leeds United for comment.
Asked about the booing, Leeds assistant manager Edmund Riemer told Sky Sports: "I'm focused on the game so I don't really hear it, but disappointed with some supporters that that happened."
Comment: Leeds fans do right to boo. muslim players, if they have to be in this country, should adapt to British ways. It shouldn't be the other way round - why should we pander to muslims who are followers of an evil cult?