Sir Keir Starmer has called on Kemi Badenoch to sack shadow justice secretary Nick Timothy after he described a mass muslim public prayer as an "act of domination and division".
Timothy was referring to the prayers which took place at an open iftar event in London's Trafalgar Square on Monday.
At prime minister's questions, Sir Keir urged Badenoch to condemn the Conservative MP's comments on social media, which he claimed showed her party had a "problem with muslims".
Badenoch said the MP was "defending British values". The Tory leader's spokesman said Timothy's comments were based on footage showing segregated males praying at the event.
London mayor Sir Sadiq Khan was among thousands to attend the Trafalgar Square open iftar organised by the Ramadan Tent Project, which says it aims to improve relations between different communities.
The charity has hosted about 20 open iftar meals across the country this year, under the theme of hope.
An iftar is a meal shared at the end of a day of fasting during Ramadan, with prayers commonly taking place before or during.
In a post on X, Timothy said: "Mass ritual prayer in public places is an act of domination."
He added: "Perform these rituals in mosques if you wish. But they are not welcome in our public places and shared institutions."
The prime minister claimed Timothy's comments were "straight from the islamist playbook".
"If he was in my team, he'd be gone. It's utterly appalling. She should denounce his comments and she should sack him," he said at PMQs.
Badenoch said Timothy was "defending British values" rather than "abolishing jury trials", drawing comparisons with Justice Secretary David Lammy's reform plans.
"I know who I would rather have sitting on the front bench next to me, and it's not him," she told the PM.
Comment: Starmer is a traitor. He doesn't care about ordinary British citizens, only muslims. The so-called religion of islam does not belong in this country. We need to begin the mass deportation of all muslims, and the dismantling of mosques. Nick Timothy is absolutely right. When will the government realise that the majority of people do not want muslims in this country. Contact you representatives now and ask them what they are doing about getting rid of these people.